[Effect of HIF-1α Gene Silence on Biological Characteristics of Human Colon Cancer Cells SW480].

ABSTRACT

OBJECTIVE: To investigate changes in proliferation and apoptosis of human colon cancer SW480 cells after silencing hypoxia inducible factor-lα (HIF-1α) expression. METHODS: siRNA interference technology was performed to silence the expression of HIF-1α using lipofectamine mediation to transfect siRNA into human colon cancer SW480 cells. The siRNA interfered SW480 cells were compared with a negative control group, an empty vector group, and a blank control group. Real-time PCR and Western blot were used to measure the expressions of HIF-lα protein and mRNA. MTT and flow cytometry (FCM) were used to evaluate the apoptosis and proliferation of SW480 cells. RESULTS: The interfered SW480 cells had a higher level of silence of HIF-lα mRNA (> 80%) compared with those of in the three control groups (P < 0.05). Higher levels of apoptosis and lower levels of proliferation were detected in the interfered cells compared with those in the control groups (P < 0.01). CONCLUSION: HIF-lα silencing promotes apoptosis and inhibits the proliferation of SW480 cells in vitro.