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Detection of mecA- and mecC-Positive Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates by the New Xpert MRSA Gen 3 PCR Assay.
Becker, Karsten; Denis, Olivier; Roisin, Sandrine; Mellmann, Alexander; Idelevich, Evgeny A; Knaack, Dennis; van Alen, Sarah; Kriegeskorte, André; Köck, Robin; Schaumburg, Frieder; Peters, Georg; Ballhausen, Britta.
Afiliação
  • Becker K; Institute of Medical Microbiology, University Hospital Münster, Münster, Germany kbecker@uni-muenster.de.
  • Denis O; Staphylococcus National Reference Centre, Erasme Hospital, Université Libre de Bruxelles, Brussels, Belgium.
  • Roisin S; Staphylococcus National Reference Centre, Erasme Hospital, Université Libre de Bruxelles, Brussels, Belgium.
  • Mellmann A; Institute of Hygiene, University Hospital Münster, Münster, Germany.
  • Idelevich EA; Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.
  • Knaack D; Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.
  • van Alen S; Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.
  • Kriegeskorte A; Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.
  • Köck R; Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.
  • Schaumburg F; Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.
  • Peters G; Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.
  • Ballhausen B; Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.
J Clin Microbiol ; 54(1): 180-4, 2016 Jan.
Article em En | MEDLINE | ID: mdl-26491186
ABSTRACT
An advanced methicillin-resistant Staphylococcus aureus (MRSA) detection PCR approach targeting SCCmec-orfX along with mecA and mecC was evaluated for S. aureus and coagulase-negative staphylococci. The possession of mecA and/or mecC was correctly confirmed in all cases. All methicillin-susceptible S. aureus strains (n = 98; including staphylococcal cassette chromosome mec element [SCCmec] remnants) and 98.1% of the MRSA strains (n = 160, including 10 mecC-positive MRSA) were accurately categorized.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções Estafilocócicas / Reação em Cadeia da Polimerase / Técnicas Bacteriológicas / Técnicas de Diagnóstico Molecular / Staphylococcus aureus Resistente à Meticilina Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções Estafilocócicas / Reação em Cadeia da Polimerase / Técnicas Bacteriológicas / Técnicas de Diagnóstico Molecular / Staphylococcus aureus Resistente à Meticilina Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article