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Intracellular mechanisms underlying lipid accumulation (white opaque substance) in gastric epithelial neoplasms: A pilot study of expression profiles of lipid-metabolism-associated genes.
Enjoji, Munechika; Kohjima, Motoyuki; Ohtsu, Kensei; Matsunaga, Kazuhisa; Murata, Yusuke; Nakamuta, Makoto; Imamura, Kentaro; Tanabe, Hiroshi; Iwashita, Akinori; Nagahama, Takashi; Yao, Kenshi.
Afiliação
  • Enjoji M; Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan.
  • Kohjima M; Department of Gastroenterology, National Hospital Organization Kyushu Medical Center, Fukuoka, Japan.
  • Ohtsu K; Department of Gastroenterology, Fukuoka University Chikushi Hospital, Fukuoka, Japan.
  • Matsunaga K; Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan.
  • Murata Y; Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan.
  • Nakamuta M; Department of Gastroenterology, National Hospital Organization Kyushu Medical Center, Fukuoka, Japan.
  • Imamura K; Department of Gastroenterology, Fukuoka University Chikushi Hospital, Fukuoka, Japan.
  • Tanabe H; Department of Pathology, Fukuoka University Chikushi Hospital, Fukuoka, Japan.
  • Iwashita A; Department of Pathology, Fukuoka University Chikushi Hospital, Fukuoka, Japan.
  • Nagahama T; Department of Gastroenterology, Fukuoka University Chikushi Hospital, Fukuoka, Japan.
  • Yao K; Department of Endoscopy, Fukuoka University Chikushi Hospital, Fukuoka, Japan.
J Gastroenterol Hepatol ; 31(4): 776-81, 2016 Apr.
Article em En | MEDLINE | ID: mdl-26513060
ABSTRACT
BACKGROUND AND

AIM:

White opaque substance (WOS) is a novel endoscopic finding in gastric neoplasms, indicating the intracellular accumulation of lipid droplets (LDs). However, gastric lipid metabolism has not been extensively investigated, even in normal mucosa. We investigated the expression profiles of lipid-metabolism-associated genes in gastric neoplasms.

METHODS:

Thirty-four patients with early gastric cancer or adenoma were enrolled in this study. Paired biopsy samples from tumor and adjacent non-tumor areas were obtained and analyzed by real-time polymerase chain reaction. Endoscopically resected specimens were evaluated histopathologically.

RESULTS:

Genes associated with ß-oxidation (peroxisome proliferator-activated receptor α, carnitine palmitoyltransferase 1A, and hydroxyacyl-CoA dehydrogenase), lipoprotein excretion (apolipoprotein B, microsomal triglyceride transfer protein, and acyl-CoAcholesterol acyltransferase 2), fatty acid transport (fatty acid-binding protein), construction of triglycerides in the endoplasmic reticulum (acyl-CoAdiacylglycerol acyltransferase 1), and LD degradation/lipolysis (comparative gene identification-58, adipose triglyceride lipase) were significantly downregulated in neoplasms compared with non-tumor areas. Pyruvate dehydrogenase lipoamide kinase isozyme 4 (negative regulator of glycolysis) and adipophilin (LD surface component) were also repressed. Conversely, expression levels of genes associated with de novo lipogenesis (sterol regulatory element-binding protein 1c, acyl-CoAdiacylglycerol acyltransferase 2) were significantly enhanced in neoplasms. There was no significant difference in gene expression levels between carcinomas and adenomas, or between WOS-positive and WOS-negative neoplasms.

CONCLUSION:

Gene expression profiles in neoplasms suggest a predominance of lipid storage (lipogenesis/LD formation) over consumption (ß-oxidation/excretion/lipolysis). Lipid accumulation and WOS in gastric epithelial neoplasms may be caused by impaired mitochondrial oxidation, lipoprotein excretion, and LD degradation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Gástricas / Neoplasias Epiteliais e Glandulares / Metabolismo dos Lipídeos / Lipogênese / Gotículas Lipídicas Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Gástricas / Neoplasias Epiteliais e Glandulares / Metabolismo dos Lipídeos / Lipogênese / Gotículas Lipídicas Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2016 Tipo de documento: Article