Interferon-γ stimulates CD14, TLR2 and TLR4 mRNA expression in gingival fibroblasts increasing responsiveness to bacterial challenge.
Arch Oral Biol
; 61: 36-43, 2016 Jan.
Article
em En
| MEDLINE
| ID: mdl-26513680
ABSTRACT
OBJECTIVE:
To investigate the potential effects of IFN-03A5 on the responsiveness of human gingival fibroblasts to bacterial challenge.DESIGN:
mRNA and protein expression of CD14, TLR2 and TLR4 in human gingival fibroblasts was detected by quantitative polymerase chain reaction (Q-PCR) and flow cytometry. The effect of preincubation with IFN-03A5 on subsequent bacterial LPS-induced expression of IL-6 and IL-8 by gingival fibroblasts was determined by ELISA. Bacterial LPS-induced IκBα degradation in human gingival fibroblasts was investigated by western blot.RESULTS:
Human gingival fibroblasts express CD14, TLR2 and TLR4 mRNAs. IFN-03A5, but not IL-103B2, induced mRNA expression of all three receptors and the expression of membrane bound CD14 protein. Pre-incubation of fibroblasts with IFN-03A5 and subsequent stimulation with Escherichia coli LPS or Porphyromonas gingivalis LPS led to increased production of IL-6 and IL-8. LPS-induced pro-inflammatory cytokine production was abrogated by a blocking antibody to CD14. Both E. coli LPS and P. gingivalis LPS induced IκBα degradation in human gingival fibroblasts.CONCLUSION:
Our data indicate that IFN-03A5 primes human gingival fibroblasts, through the upregulation of CD14 expression, which results in increased responsiveness to bacterial LPS challenge, as determined by pro-inflammatory cytokine production.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
/
Lipopolissacarídeos
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Interferon gama
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Receptores de Lipopolissacarídeos
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Receptor 2 Toll-Like
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Receptor 4 Toll-Like
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Fibroblastos
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Gengiva
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article