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Immunohistochemical examination of cholecystokinin and gastrin receptors (CCK-2/gastrin-R) expression in normal and exocrine cancerous human pancreatic tissues.
Sellam, Feriel; Harir, Noria; Khaled, Méghit B; Mrabent, Nesrine M; Belkralladi, Houria; Tou, Abdelnacer; Diaf, Mustapha; Salah, Rachida; Moulessehoul, Soraya.
Afiliação
  • Sellam F; Department of Biology, Djillali Liabes University of Sidi bel Abbes, Algeria. Electronic address: mayflowerboat@live.fr.
  • Harir N; Department of Biology, Djillali Liabes University of Sidi bel Abbes, Algeria; Laboratory of Molecular Microbiology, Proteomics and Health, Algeria.
  • Khaled MB; Department of Biology, Djillali Liabes University of Sidi bel Abbes, Algeria.
  • Mrabent NM; Department of Biology, Djillali Liabes University of Sidi bel Abbes, Algeria.
  • Belkralladi H; Department of Pathology, Hassani Abdelkader University Hospital, Sidi bel Abbes, Algeria.
  • Tou A; Department of Pathology, Hassani Abdelkader University Hospital, Sidi bel Abbes, Algeria; Laboratory of Environment and Cancer, Algeria.
  • Diaf M; Department of Biology, Djillali Liabes University of Sidi bel Abbes, Algeria.
  • Salah R; Department of Biology, Djillali Liabes University of Sidi bel Abbes, Algeria.
  • Moulessehoul S; Department of Biology, Djillali Liabes University of Sidi bel Abbes, Algeria.
Pancreatology ; 15(6): 661-6, 2015.
Article em En | MEDLINE | ID: mdl-26520651
OBJECTIVE: Evaluating tissue samples of normal and exocrine cancerous human pancreas on the expression of CCK2/gastrin receptor. We performed an immunohistochemical protocol that allows efficient detection of this receptor in formalin-fixed, paraffin-embedded human tissues. METHODS: Twenty (20) paraffin blocks of pancreatic tissue sections were collected from the Departments of pathology, Central University Hospital of Sidi-bel-Abbes City (Western Algeria) for the period 2004-2013; ten (10) of them were normal pancreatic samples; and ten (10) cancerous pancreatic sections. The samples were studied using an immunohistochemical protocol for CCK-2/gastrin receptors. RESULTS: Our immunohistochemical analysis revealed that CCK-2/gastrin receptors were expressed in both normal and malignant pancreatic cells but with different immunoreactivity levels and different immunostaining intensity i.e., CCK-2/gastrin receptors were highly expressed within the cytoplasmic area of cancerous cells; 40% of the samples had an immunoreactivity (IR) of (+++) and 60% (++++); the immunostaining was as well very intense since we reported a dark brown staining of the malignant cells. However; in normal pancreatic tissues; CCK-2/gastrin receptors IR levels were very low; 80% of the samples had an IR of (+); and 20% had (++) and the immunostaining was less intense; we noted a light brown staining of few normal pancreatic cells. CONCLUSION: The gastrointestinal peptides CCK could be very interesting targets for exocrine pancreatic cancer therapies; thus further surveys such as western blotting and RTPCR could indentify CCK-2/gastrin receptors as a helpful biomarker for exocrine pancreatic cancer diagnosis and treatment.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pâncreas / Neoplasias Pancreáticas / Regulação Neoplásica da Expressão Gênica / Receptor de Colecistocinina B Tipo de estudo: Guideline Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pâncreas / Neoplasias Pancreáticas / Regulação Neoplásica da Expressão Gênica / Receptor de Colecistocinina B Tipo de estudo: Guideline Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2015 Tipo de documento: Article