Rhizobium acidisoli sp. nov., isolated from root nodules of Phaseolus vulgaris in acid soils.
Int J Syst Evol Microbiol
; 66(1): 398-406, 2016 01.
Article
em En
| MEDLINE
| ID: mdl-26530784
ABSTRACT
Two Gram-negative, aerobic, non-motile, rod-shaped bacterial strains, FH13T and FH23, representing a novel group of Rhizobium isolated from root nodules of Phaseolus vulgaris in Mexico, were studied by a polyphasic analysis. Phylogeny of 16S rRNA gene sequences revealed them to be members of the genus Rhizobium related most closely to 'Rhizobium anhuiense' CCBAU 23252 (99.7 % similarity), Rhizobium leguminosarum USDA 2370T (98.6 %), and Rhizobium sophorae CCBAU 03386T and others ( ≤ 98.3 %). In sequence analyses of the housekeeping genes recA, glnII and atpD, both strains formed a subclade distinct from all defined species of the genus Rhizobium at sequence similarities of 82.3-94.0 %, demonstrating that they represented a novel genomic species in the genus Rhizobium. Mean levels of DNA-DNA relatedness between the reference strain FH13T and the type strains of related species varied between 13.0 ± 2.0 and 52.1 ± 1.2 %. The DNA G+C content of strain FH13T was 63.5âmol% (Tm). The major cellular fatty acids were 16 0, 17 0 anteiso, 18 0, summed feature 2 (12 0 aldehyde/unknown 10.928) and summed feature 8 (18 1ω7c). The fatty acid 17 1ω5c was unique for this strain. Some phenotypic features, such as failure to utilize adonitol, l-arabinose, d-fructose and d-fucose, and ability to utilize d-galacturonic acid and itaconic acid as carbon source, could also be used to distinguish strain FH13T from the type strains of related species. Based upon these results, a novel species, Rhizobium acidisoli sp. nov., is proposed, with FH13T ( = CCBAU 101094T = HAMBI 3626T = LMG 28672T) as the type strain.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
Rhizobium
/
Phaseolus
/
Nódulos Radiculares de Plantas
País/Região como assunto:
Mexico
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article