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Overexpression of the olive acyl carrier protein gene (OeACP1) produces alterations in fatty acid composition of tobacco leaves.
De Marchis, Francesca; Valeri, Maria Cristina; Pompa, Andrea; Bouveret, Emmanuelle; Alagna, Fiammetta; Grisan, Simone; Stanzione, Vitale; Mariotti, Roberto; Cultrera, Nicolò; Baldoni, Luciana; Bellucci, Michele.
Afiliação
  • De Marchis F; Institute of Biosciences and Bioresources (IBBR), Research Division of Perugia, CNR, Via Madonna Alta 130, 06128, Perugia, Italy.
  • Valeri MC; Institute of Biosciences and Bioresources (IBBR), Research Division of Perugia, CNR, Via Madonna Alta 130, 06128, Perugia, Italy.
  • Pompa A; PlantLab, Institute of Life Sciences, Scuola Superiore Sant'Anna, 56127, Pisa, Italy.
  • Bouveret E; Institute of Biosciences and Bioresources (IBBR), Research Division of Perugia, CNR, Via Madonna Alta 130, 06128, Perugia, Italy.
  • Alagna F; LISM, CNRS, Aix-Marseille University, 13402, Marseille Cedex 20, France.
  • Grisan S; Institute of Biosciences and Bioresources (IBBR), Research Division of Perugia, CNR, Via Madonna Alta 130, 06128, Perugia, Italy.
  • Stanzione V; Research Unit for Table Grapes and Wine Growing in Mediterranean Environment, CREA, Via Casamassima 148, Turi, 70010, Bari, Italy.
  • Mariotti R; Institute of Biosciences and Bioresources (IBBR), Research Division of Perugia, CNR, Via Madonna Alta 130, 06128, Perugia, Italy.
  • Cultrera N; Institute for Agricultural and Forest Systems in the Mediterranean (ISAFOM), Research Division of Perugia, CNR, Via Madonna Alta 128, 06128, Perugia, Italy.
  • Baldoni L; Institute of Biosciences and Bioresources (IBBR), Research Division of Perugia, CNR, Via Madonna Alta 130, 06128, Perugia, Italy.
  • Bellucci M; Institute of Biosciences and Bioresources (IBBR), Research Division of Perugia, CNR, Via Madonna Alta 130, 06128, Perugia, Italy.
Transgenic Res ; 25(1): 45-61, 2016 Feb.
Article em En | MEDLINE | ID: mdl-26560313
Taking into account that fatty acid (FA) biosynthesis plays a crucial role in lipid accumulation in olive (Olea europaea L.) mesocarp, we investigated the effect of olive acyl carrier protein (ACP) on FA composition by overexpressing an olive ACP cDNA in tobacco plants. The OeACP1.1A cDNA was inserted in the nucleus or in the chloroplast DNA of different tobacco plants, resulting in extensive transcription of the transgenes. The transplastomic plants accumulated lower olive ACP levels in comparison to nuclear-transformed plants. Moreover, the phenotype of the former plants was characterized by pale green/white cotyledons with abnormal chloroplasts, delayed germination and reduced growth. We suggest that the transplastomic phenotype was likely caused by inefficient olive ACP mRNA translation in chloroplast stroma. Conversely, total lipids from leaves of nuclear transformants expressing high olive ACP levels showed a significant increase in oleic acid (18:1) and linolenic acid (18:3), and a concomitant significant reduction of hexadecadienoic acid (16:2) and hexadecatrienoic acid (16:3). This implies that in leaves of tobacco transformants, as likely in the mesocarp of olive fruit, olive ACP not only plays a general role in FA synthesis, but seems to be specifically involved in chain length regulation forwarding the elongation to C18 FAs and the subsequent desaturation to 18:1 and 18:3.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nicotiana / Proteína de Transporte de Acila / Folhas de Planta / Olea / Ácidos Graxos Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nicotiana / Proteína de Transporte de Acila / Folhas de Planta / Olea / Ácidos Graxos Idioma: En Ano de publicação: 2016 Tipo de documento: Article