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GAR22ß regulates cell migration, sperm motility, and axoneme structure.
Gamper, Ivonne; Fleck, David; Barlin, Meltem; Spehr, Marc; El Sayad, Sara; Kleine, Henning; Maxeiner, Sebastian; Schalla, Carmen; Aydin, Gülcan; Hoss, Mareike; Litchfield, David W; Lüscher, Bernhard; Zenke, Martin; Sechi, Antonio.
Afiliação
  • Gamper I; Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Medical School, D-52074 Aachen, Germany Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, D-52074 Aachen, Germany.
  • Fleck D; Institute for Biology II, Department of Chemosensation, RWTH Aachen University, D-52074 Aachen, Germany.
  • Barlin M; Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Medical School, D-52074 Aachen, Germany Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, D-52074 Aachen, Germany.
  • Spehr M; Institute for Biology II, Department of Chemosensation, RWTH Aachen University, D-52074 Aachen, Germany.
  • El Sayad S; Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Medical School, D-52074 Aachen, Germany Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, D-52074 Aachen, Germany.
  • Kleine H; Institute of Biochemistry and Molecular Biology, Uniklinik RWTH Aachen, D-52074 Aachen, Germany.
  • Maxeiner S; Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Medical School, D-52074 Aachen, Germany Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, D-52074 Aachen, Germany.
  • Schalla C; Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Medical School, D-52074 Aachen, Germany Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, D-52074 Aachen, Germany.
  • Aydin G; Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Medical School, D-52074 Aachen, Germany Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, D-52074 Aachen, Germany.
  • Hoss M; Electron Microscopy Facility, Uniklinik RWTH Aachen, D-52074 Aachen, Germany.
  • Litchfield DW; Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON N6A 5C1, Canada.
  • Lüscher B; Institute of Biochemistry and Molecular Biology, Uniklinik RWTH Aachen, D-52074 Aachen, Germany.
  • Zenke M; Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Medical School, D-52074 Aachen, Germany Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, D-52074 Aachen, Germany.
  • Sechi A; Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Medical School, D-52074 Aachen, Germany Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, D-52074 Aachen, Germany Antonio.Sechi@rwth-aachen.de.
Mol Biol Cell ; 27(2): 277-94, 2016 Jan 15.
Article em En | MEDLINE | ID: mdl-26564797
Spatiotemporal cytoskeleton remodeling is pivotal for cell adhesion and migration. Here we investigated the function of Gas2-related protein on chromosome 22 (GAR22ß), a poorly characterized protein that interacts with actin and microtubules. Primary and immortalized GAR22ß(-/-) Sertoli cells moved faster than wild-type cells. In addition, GAR22ß(-/-) cells showed a more prominent focal adhesion turnover. GAR22ß overexpression or its reexpression in GAR22ß(-/-) cells reduced cell motility and focal adhesion turnover. GAR22ß-actin interaction was stronger than GAR22ß-microtubule interaction, resulting in GAR22ß localization and dynamics that mirrored those of the actin cytoskeleton. Mechanistically, GAR22ß interacted with the regulator of microtubule dynamics end-binding protein 1 (EB1) via a novel noncanonical amino acid sequence, and this GAR22ß-EB1 interaction was required for the ability of GAR22ß to modulate cell motility. We found that GAR22ß is highly expressed in mouse testes, and its absence resulted in reduced spermatozoa generation, lower actin levels in testes, and impaired motility and ultrastructural disorganization of spermatozoa. Collectively our findings identify GAR22ß as a novel regulator of cell adhesion and migration and provide a foundation for understanding the molecular basis of diverse cytoskeleton-dependent processes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Motilidade dos Espermatozoides / Movimento Celular / Proteínas dos Microfilamentos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2016 Tipo de documento: Article
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Motilidade dos Espermatozoides / Movimento Celular / Proteínas dos Microfilamentos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2016 Tipo de documento: Article