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The genetic diversity of Epstein-Barr virus in the setting of transplantation relative to non-transplant settings: A feasibility study.
Allen, Upton D; Hu, Pingzhao; Pereira, Sergio L; Robinson, Joan L; Paton, Tara A; Beyene, Joseph; Khodai-Booran, Nasser; Dipchand, Anne; Hébert, Diane; Ng, Vicky; Nalpathamkalam, Thomas; Read, Stanley.
Afiliação
  • Allen UD; Division of Infectious Diseases, Department of Paediatrics, Hospital for Sick Children, University of Toronto, Toronto, ON, Canada.
  • Hu P; Research Institute, Hospital for Sick Children, University of Toronto, Toronto, ON, Canada.
  • Pereira SL; The Transplant and Regenerative Medicine Centre, Hospital for Sick Children, University of Toronto, Toronto, ON, Canada.
  • Robinson JL; The Centre for Applied Genomics, Hospital for Sick Children, Toronto, ON, Canada.
  • Paton TA; The Centre for Applied Genomics, Hospital for Sick Children, Toronto, ON, Canada.
  • Beyene J; Division of Infectious Diseases, Department of Paediatrics, Stollery Children's Hospital, Edmonton, AB, Canada.
  • Khodai-Booran N; The Centre for Applied Genomics, Hospital for Sick Children, Toronto, ON, Canada.
  • Dipchand A; Research Institute, Hospital for Sick Children, University of Toronto, Toronto, ON, Canada.
  • Hébert D; Research Institute, Hospital for Sick Children, University of Toronto, Toronto, ON, Canada.
  • Ng V; The Transplant and Regenerative Medicine Centre, Hospital for Sick Children, University of Toronto, Toronto, ON, Canada.
  • Nalpathamkalam T; The Transplant and Regenerative Medicine Centre, Hospital for Sick Children, University of Toronto, Toronto, ON, Canada.
  • Read S; The Transplant and Regenerative Medicine Centre, Hospital for Sick Children, University of Toronto, Toronto, ON, Canada.
Pediatr Transplant ; 20(1): 124-9, 2016 Feb.
Article em En | MEDLINE | ID: mdl-26578436
ABSTRACT
This study examines EBV strains from transplant patients and patients with IM by sequencing major EBV genes. We also used NGS to detect EBV DNA within total genomic DNA, and to evaluate its genetic variation. Sanger sequencing of major EBV genes was used to compare SNVs from samples taken from transplant patients vs. patients with IM. We sequenced EBV DNA from a healthy EBV-seropositive individual on a HiSeq 2000 instrument. Data were mapped to the EBV reference genomes (AG876 and B95-8). The number of EBNA2 SNVs was higher than for EBNA1 and the other genes sequenced within comparable reference coordinates. For EBNA2, there was a median of 15 SNV among transplant samples compared with 10 among IM samples (p = 0.036). EBNA1 showed little variation between samples. For NGS, we identified 640 and 892 variants at an unadjusted p value of 5 × 10(-8) for AG876 and B95-8 genomes, respectively. We used complementary sequence strategies to examine EBV genetic diversity and its application to transplantation. The results provide the framework for further characterization of EBV strains and related outcomes after organ transplantation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Herpesvirus Humano 4 / Mononucleose Infecciosa Tipo de estudo: Etiology_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Adolescent / Adult / Child / Child, preschool / Humans / Infant Idioma: En Ano de publicação: 2016 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Herpesvirus Humano 4 / Mononucleose Infecciosa Tipo de estudo: Etiology_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Adolescent / Adult / Child / Child, preschool / Humans / Infant Idioma: En Ano de publicação: 2016 Tipo de documento: Article