Influence of lipidation on the mode of action of a small RW-rich antimicrobial peptide.
Biochim Biophys Acta
; 1858(5): 1004-11, 2016 May.
Article
em En
| MEDLINE
| ID: mdl-26603779
ABSTRACT
Antimicrobial peptides are a potent class of antibiotics. In the Gram-positive model organism Bacillus subtilis the synthetic peptide RWRWRW-NH2 integrates into the bacterial membrane and delocalizes essential peripheral membrane proteins involved in cell wall biosynthesis and respiration. A lysine residue has been added to the hexapeptide core structure, either C or N-terminally. Lipidation of the lysine residues by a C8-acyl chain significantly improved antibacterial activity against both Gram-positive and Gram-negative bacteria. Here, we report a comparative proteomic study in B. subtilis on the mechanism of action of the lipidated and non-lipidated peptides. All derivatives depolarized the bacterial membrane without forming pores and all affected cell wall integrity. Proteomic profiling of the bacterial stress responses to the small RW-rich antimicrobial peptides was reflective of non-disruptive membrane integration. Overall, our results indicate that antimicrobial peptides can be derivatized with lipid chains enhancing antibacterial activity without significantly altering the mechanism of action. This article is part of a Special Issue entitled Antimicrobial peptides edited by Karl Lohner and Kai Hilpert.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bacillus subtilis
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Regulação Bacteriana da Expressão Gênica
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Membrana Celular
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Parede Celular
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Peptídeos Catiônicos Antimicrobianos
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Escherichia coli
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Antibacterianos
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article