Extended HSR/CARD domain mediates AIRE binding to DNA.
Biochem Biophys Res Commun
; 468(4): 913-20, 2015 Dec 25.
Article
em En
| MEDLINE
| ID: mdl-26607109
ABSTRACT
Autoimmune regulator (AIRE) activates the transcription of many genes in an unusual promiscuous and stochastic manner. The mechanism by which AIRE binds to the chromatin and DNA is not fully understood, and the regulatory elements that AIRE target genes possess are not delineated. In the current study, we demonstrate that AIRE activates the expression of transiently transfected luciferase reporters that lack defined promoter regions, as well as intron and poly(A) signal sequences. Our protein-DNA interaction experiments with mutated AIRE reveal that the intact homogeneously staining region/caspase recruitment domain (HSR/CARD) and amino acids R113 and K114 are key elements involved in AIRE binding to DNA.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
DNA
/
Proteínas Adaptadoras de Sinalização CARD
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article