Genome Modification of Pluripotent Cells by Using Transcription Activator-Like Effector Nucleases (TALENs).
Methods Mol Biol
; 1330: 253-67, 2015.
Article
em En
| MEDLINE
| ID: mdl-26621602
ABSTRACT
Interest is increasing in transcription activator-like effector nucleases (TALENs) as a tool to introduce targeted double-strand breaks into the large genomes of human and animal cell lines. The produced DNA lesions stimulate DNA repair pathways, error-prone but dominant non-homologous end joining (NHEJ) and accurate but less occurring homology-directed repair (HDR), and as a result targeted genes can be modified. Here, we describe a modified Golden-Gate cloning method for generating TALENs and also details for targeting genes in mouse embryonic stem cells. The protocol described here can be used for modifying the genome of a broad range of pluripotent cell lines.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Engenharia Genética
/
Genoma
/
Marcação de Genes
/
Endonucleases
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article