Prevention of Dielectric Breakdown of Nanopore Membranes by Charge Neutralization.
Sci Rep
; 5: 17819, 2015 Dec 04.
Article
em En
| MEDLINE
| ID: mdl-26634995
ABSTRACT
To achieve DNA sequencing using a solid-state nanopore, it is necessary to reduce the electric noise current. The noise current can be decreased by reducing the capacitance (C) of the nanopore device. However, we found that an electric-charge difference (ΔQ) between the electrolyte in one chamber and the electrolyte in another chamber occurred. For low capacitance devices, this electric-charge imbalance can lead to unexpectedly high voltage (ΔV = ΔQ/C) which disrupted the membrane when the two electrolytes were independently poured into the chambers. We elucidated the mechanism for the generation of initial defects and established new procedures for preventing the generation of defects by connecting an electric bypass between the chambers.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Análise de Sequência de DNA
/
Nanotecnologia
/
Nanoporos
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article