[Rapid Identification Method Positive Blood Culture Bottles with MALDI Biotyper].
Rinsho Biseibutshu Jinsoku Shindan Kenkyukai Shi
; 26(1): 13-9, 2015.
Article
em Ja
| MEDLINE
| ID: mdl-26635004
ABSTRACT
Identification method positive blood culture bottles with MALDI Biotyper is the most important test on precisely and rapidly, for detamination the bacterial name in sepsis and bacteremia is very significant for decision a cure. This time, we devised a new method "blend" to identify the mixture hypostasis that were come into being by centrifuging blood culture broths and 70% formic acid with MALDI Biotyper (Bruker). This time, we identified 65 samples rapidly with MALDI biotyper by "on plate" and "blend," and verified their effectivity. As a result of six ways (on plate, blend-3, blend-6, blend-9, blend-12, blend-15), the highest detection rate was Gram negative rods blend-15 (74.1%), Gram positive cocci blend-9 (56.3%), total blend-9 (55.4%). Moreover, we confirmed that the detection rate raised to 85.2% (GNR), 71.0% (GPC) and 77.6% (total), and the usefully was suggested. Our invented method is more excellently than recommended on Gram negative rods, especially Enterobacteriaceae, Staphylococcus aureus, and Enterococcus spp.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bacteriemia
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
Ja
Ano de publicação:
2015
Tipo de documento:
Article