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Revisiting Cardiac Cellular Composition.
Pinto, Alexander R; Ilinykh, Alexei; Ivey, Malina J; Kuwabara, Jill T; D'Antoni, Michelle L; Debuque, Ryan; Chandran, Anjana; Wang, Lina; Arora, Komal; Rosenthal, Nadia A; Tallquist, Michelle D.
Afiliação
  • Pinto AR; From the Australian Regenerative Medicine Institute, Monash University, Melbourne, Victoria, Australia (A.R.P., A.I., R.D., A.C., L.W., N.R.); Department of Medicine, Center for Cardiovascular Research (M.J.I., J.T.K., M.L.D'A., K.A., M.D.T.) and Department of Cellular and Molecular Biology (M.J.I.,
  • Ilinykh A; From the Australian Regenerative Medicine Institute, Monash University, Melbourne, Victoria, Australia (A.R.P., A.I., R.D., A.C., L.W., N.R.); Department of Medicine, Center for Cardiovascular Research (M.J.I., J.T.K., M.L.D'A., K.A., M.D.T.) and Department of Cellular and Molecular Biology (M.J.I.,
  • Ivey MJ; From the Australian Regenerative Medicine Institute, Monash University, Melbourne, Victoria, Australia (A.R.P., A.I., R.D., A.C., L.W., N.R.); Department of Medicine, Center for Cardiovascular Research (M.J.I., J.T.K., M.L.D'A., K.A., M.D.T.) and Department of Cellular and Molecular Biology (M.J.I.,
  • Kuwabara JT; From the Australian Regenerative Medicine Institute, Monash University, Melbourne, Victoria, Australia (A.R.P., A.I., R.D., A.C., L.W., N.R.); Department of Medicine, Center for Cardiovascular Research (M.J.I., J.T.K., M.L.D'A., K.A., M.D.T.) and Department of Cellular and Molecular Biology (M.J.I.,
  • D'Antoni ML; From the Australian Regenerative Medicine Institute, Monash University, Melbourne, Victoria, Australia (A.R.P., A.I., R.D., A.C., L.W., N.R.); Department of Medicine, Center for Cardiovascular Research (M.J.I., J.T.K., M.L.D'A., K.A., M.D.T.) and Department of Cellular and Molecular Biology (M.J.I.,
  • Debuque R; From the Australian Regenerative Medicine Institute, Monash University, Melbourne, Victoria, Australia (A.R.P., A.I., R.D., A.C., L.W., N.R.); Department of Medicine, Center for Cardiovascular Research (M.J.I., J.T.K., M.L.D'A., K.A., M.D.T.) and Department of Cellular and Molecular Biology (M.J.I.,
  • Chandran A; From the Australian Regenerative Medicine Institute, Monash University, Melbourne, Victoria, Australia (A.R.P., A.I., R.D., A.C., L.W., N.R.); Department of Medicine, Center for Cardiovascular Research (M.J.I., J.T.K., M.L.D'A., K.A., M.D.T.) and Department of Cellular and Molecular Biology (M.J.I.,
  • Wang L; From the Australian Regenerative Medicine Institute, Monash University, Melbourne, Victoria, Australia (A.R.P., A.I., R.D., A.C., L.W., N.R.); Department of Medicine, Center for Cardiovascular Research (M.J.I., J.T.K., M.L.D'A., K.A., M.D.T.) and Department of Cellular and Molecular Biology (M.J.I.,
  • Arora K; From the Australian Regenerative Medicine Institute, Monash University, Melbourne, Victoria, Australia (A.R.P., A.I., R.D., A.C., L.W., N.R.); Department of Medicine, Center for Cardiovascular Research (M.J.I., J.T.K., M.L.D'A., K.A., M.D.T.) and Department of Cellular and Molecular Biology (M.J.I.,
  • Rosenthal NA; From the Australian Regenerative Medicine Institute, Monash University, Melbourne, Victoria, Australia (A.R.P., A.I., R.D., A.C., L.W., N.R.); Department of Medicine, Center for Cardiovascular Research (M.J.I., J.T.K., M.L.D'A., K.A., M.D.T.) and Department of Cellular and Molecular Biology (M.J.I.,
  • Tallquist MD; From the Australian Regenerative Medicine Institute, Monash University, Melbourne, Victoria, Australia (A.R.P., A.I., R.D., A.C., L.W., N.R.); Department of Medicine, Center for Cardiovascular Research (M.J.I., J.T.K., M.L.D'A., K.A., M.D.T.) and Department of Cellular and Molecular Biology (M.J.I.,
Circ Res ; 118(3): 400-9, 2016 Feb 05.
Article em En | MEDLINE | ID: mdl-26635390
RATIONALE: Accurate knowledge of the cellular composition of the heart is essential to fully understand the changes that occur during pathogenesis and to devise strategies for tissue engineering and regeneration. OBJECTIVE: To examine the relative frequency of cardiac endothelial cells, hematopoietic-derived cells, and fibroblasts in the mouse and human heart. METHODS AND RESULTS: Using a combination of genetic tools and cellular markers, we examined the occurrence of the most prominent cell types in the adult mouse heart. Immunohistochemistry revealed that endothelial cells constitute >60%, hematopoietic-derived cells 5% to 10%, and fibroblasts <20% of the nonmyocytes in the heart. A refined cell isolation protocol and an improved flow cytometry approach provided an independent means of determining the relative abundance of nonmyocytes. High-dimensional analysis and unsupervised clustering of cell populations confirmed that endothelial cells are the most abundant cell population. Interestingly, fibroblast numbers are smaller than previously estimated, and 2 commonly assigned fibroblast markers, Sca-1 and CD90, under-represent fibroblast numbers. We also describe an alternative fibroblast surface marker that more accurately identifies the resident cardiac fibroblast population. CONCLUSIONS: This new perspective on the abundance of different cell types in the heart demonstrates that fibroblasts comprise a relatively minor population. By contrast, endothelial cells constitute the majority of noncardiomyocytes and are likely to play a greater role in physiological function and response to injury than previously appreciated.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco Hematopoéticas / Células Endoteliais / Fibroblastos / Coração Limite: Adult / Animals / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco Hematopoéticas / Células Endoteliais / Fibroblastos / Coração Limite: Adult / Animals / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2016 Tipo de documento: Article