New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters.
Nat Prod Rep
; 33(2): 174-82, 2016 Feb.
Article
em En
| MEDLINE
| ID: mdl-26647833
ABSTRACT
Natural product scaffolds remain a major source and inspiration for human therapeutics. However, generation of a natural product in the post-genomic era often requires reconstruction of the corresponding biosynthetic gene cluster in a heterologous host. In the burgeoning fields of synthetic biology and metabolic engineering, a significant amount of efforts has been devoted to develop DNA assembly techniques with higher efficiency, fidelity, and modularity, and heterologous expression systems with higher productivity and yield. Here we describe recent advances in DNA assembly and host engineering and highlight their applications in natural product discovery and engineering.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Produtos Biológicos
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article