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Different patterns of allelic imbalance in sporadic tumors and tumors associated with long-term exposure to gamma-radiation.
Litviakov, Nikolai V; Freidin, Maxim B; Sazonov, Aleksey E; Khalyuzova, Maria V; Buldakov, Mikhail A; Karbyshev, Mikhail S; Albakh, Еlena N; Isubakova, Daria S; Gagarin, Аleksey A; Nekrasov, Gennadiy B; Mironova, Elena B; Izosimov, Аndrey S; Takhauov, Ravil M; Karpov, Аndrei B.
Afiliação
  • Litviakov NV; Seversk Biophysical Research Centre of the Federal Medical and Biological Agency, Seversk, Russia; Tomsk Cancer Research Institute, Tomsk, Russia; National Research Tomsk State University, Tomsk, Russia. Electronic address: nvlitv72@yandex.ru.
  • Freidin MB; Population Genetics Laboratory, Research Institute for Medical Genetics, Tomsk, Russia.
  • Sazonov AE; Seversk Biophysical Research Centre of the Federal Medical and Biological Agency, Seversk, Russia.
  • Khalyuzova MV; Seversk Biophysical Research Centre of the Federal Medical and Biological Agency, Seversk, Russia; National Research Tomsk State University, Tomsk, Russia.
  • Buldakov MA; Tomsk Cancer Research Institute, Tomsk, Russia; National Research Tomsk State University, Tomsk, Russia.
  • Karbyshev MS; Tomsk Cancer Research Institute, Tomsk, Russia; National Research Tomsk State University, Tomsk, Russia.
  • Albakh ЕN; Seversk Biophysical Research Centre of the Federal Medical and Biological Agency, Seversk, Russia.
  • Isubakova DS; Seversk Biophysical Research Centre of the Federal Medical and Biological Agency, Seversk, Russia.
  • Gagarin АA; Clinical Hospital #81 of the Federal Medical and Biological Agency, Seversk, Russia.
  • Nekrasov GB; Clinical Hospital #81 of the Federal Medical and Biological Agency, Seversk, Russia.
  • Mironova EB; Clinical Hospital #81 of the Federal Medical and Biological Agency, Seversk, Russia.
  • Izosimov АS; Clinical Hospital #81 of the Federal Medical and Biological Agency, Seversk, Russia.
  • Takhauov RM; Seversk Biophysical Research Centre of the Federal Medical and Biological Agency, Seversk, Russia.
  • Karpov АB; Seversk Biophysical Research Centre of the Federal Medical and Biological Agency, Seversk, Russia.
Article em En | MEDLINE | ID: mdl-26653978
ABSTRACT
The study aimed to reveal cancer related mutations in DNA repair and cell cycle genes associated with chronic occupational exposure to gamma-radiation in personnel of the Siberian Group of Chemical Enterprises (SGCE). Mutations were analyzed by comparing genotypes of malignant tumors and matched normal tissues of 255 cancer patients including 98 exposed to external gamma-radiation (mean dose 128.1±150.5mSv). Also a genetic association analysis was carried out in a sample of 149 cancer patients and 908 healthy controls occupationally exposed to gamma-radiation (153.2±204.6mSv and 150.5±211.2mSv, respectively). Eight SNPs of genes of DNA excision repair were genotyped (rs13181, rs1052133, rs1042522, rs2305427, rs4244285, rs1045642, rs1805419 and rs1801133). The mutation profiles in heterozygous loci for selected SNP were different between sporadic tumors and tumors in patients exposed to radiation. In sporadic tumors, heterozygous genotype Arg/Pro of the rs1042522 SNP mutated into Arg/0 in 15 cases (9.6%) and 0/Pro in 14 cases (8.9%). The genotype Lys/Gln of the rs13181 SNP mutated into Lys/0 and 0/Gln in 9 and 4 cases, respectively. In tumors of patients exposed to low-level radiation, the rs1042522 Arg/0 mutated genotype was found in 12 cases (12.1%), while in 2 cases (2%) 0/Pro mutation was observed. Finally, the rs13181 0/Gln mutated genotype was observed in 15 cases (16,5%) . Thus, our study showed the difference in patterns of allelic imbalance in tumors appeared under low-level radiation exposure and spontaneous tumors for selected SNPs. This suggests different mechanisms of inactivation of heterozygous genotypes in sporadic and radiation-induced tumors.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Exposição Ocupacional / Desequilíbrio Alélico / Raios gama / Neoplasias Tipo de estudo: Etiology_studies / Observational_studies / Risk_factors_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Exposição Ocupacional / Desequilíbrio Alélico / Raios gama / Neoplasias Tipo de estudo: Etiology_studies / Observational_studies / Risk_factors_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2015 Tipo de documento: Article