Annular dark field transmission electron microscopy for protein structure determination.
Ultramicroscopy
; 161: 98-104, 2016 Feb.
Article
em En
| MEDLINE
| ID: mdl-26656466
ABSTRACT
Recently annular dark field (ADF) transmission electron microscopy (TEM) has been advocated as a means of recording images of biological specimens with better signal to noise ratio (SNR) than regular bright field images. I investigate whether and how such images could be used to determine the three-dimensional structure of proteins given that an ADF aperture with a suitable pass-band can be manufactured and used in practice. I develop an approximate theory of ADF-TEM image formation for weak amplitude and phase objects and test this theory using computer simulations. I also test whether these simulated images can be used to calculate a three-dimensional model of the protein using standard software and discuss problems and possible ways to overcome these.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Microscopia Eletrônica de Transmissão
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article