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Label-free colorimetric detection of biological thiols based on target-triggered inhibition of photoinduced formation of AuNPs.
Jung, Ye Lim; Park, Jung Hun; Kim, Moon Il; Park, Hyun Gyu.
Afiliação
  • Jung YL; Department of Chemical and Biomolecular Engineering (BK21+ Program), KAIST, Daejeon 305-701, Korea. Department of Business Opportunity Analysis, Technology Innovation Analysis Center, Korea Institute of Science and Technology Information (KISTI), Seoul 130-741, Korea.
Nanotechnology ; 27(5): 055501, 2016 Feb 05.
Article em En | MEDLINE | ID: mdl-26671249
ABSTRACT
A label-free colorimetric method for the detection of biological thiols (biothiols) was developed. This method is based on prevention of the photoinduced reduction of auric ions (Au(III)) in the presence of amino acids (acting as a reducing agent) by biothiols; the photoinduced reduction is inhibited due to the strong interaction of the biothiols with Au(III). In this method, the sample was first incubated in an assay solution containing Au(III) and threonine; the sample solution was then exposed to 254 nm UV light. For samples without biothiols, this process led to the photoreduction of Au(III) followed by growth of gold nanoparticles accompanied by the visually detectable development of a red coloration typified by an absorption peak at ca 530 nm. Conversely, in the presence of biothiols, reduction of Au(III) to Au(0) was prevented by entrapment of Au(III) within the biothiols via the thiol group. The solution thus remained colorless even after UV irradiation, which was used as an indicator of the presence of biothiols. Using this strategy, biothiols were very conveniently analyzed by monitoring color changes of the samples with the naked eye or a UV-vis spectrometer. The strategy based on this interesting phenomenon exhibited high selectivity toward biothiols over common amino acids and was successfully employed for reliable quantification of biothiols present in human plasma, demonstrating its great potential for clinical applications.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Compostos de Sulfidrila / Técnicas Biossensoriais / Colorimetria / Nanopartículas Metálicas / Ouro Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Compostos de Sulfidrila / Técnicas Biossensoriais / Colorimetria / Nanopartículas Metálicas / Ouro Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article