Morphological and molecular characterization of Oestromyia leporina (Pallas, 1778) (Diptera: Hypodermatinae) from wild plateau pikas(Ochotona curzoniae) in Qinghai province, China.
Exp Parasitol
; 161: 27-34, 2016 Feb.
Article
em En
| MEDLINE
| ID: mdl-26708934
ABSTRACT
The objective of this article was to investigate the morphological and molecular characterization of Oestromyia leporina (Pallas, 1778) from wild plateau pikas (Ochotona curzoniae) in Qinghai province, China. The third-stage larvae of O. leporina were examined by scanning electron microscopy revealing morphology characteristics of the spines on the cephalic, the thoracic segments, the abdominal segments and the spiracular plates. The coding regions of 25 cytochrome oxidase I (COI) genes of O. leporina were investigated. Eighty-one variable sites and 21 haplotypes were identified and the nucleotide and haplotype diversities were 0.04456 and 0.9767, respectively, indicating a rich genetic diversity in O. leporina. Phylogenetic analysis utilizing sequences of COI revealed two distinct lineages. These findings revealed ultrastructure and molecular characterization among the O. leporina from plateau pikas in Qinghai province, China and had implications for studying morphological identification, molecular epidemiology and population genetics of O. leporina.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Ribossômico 18S
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Dípteros
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Ectoparasitoses
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Lagomorpha
Tipo de estudo:
Prognostic_studies
Limite:
Animals
País/Região como assunto:
Asia
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article