In Vitro Validation of Intratumoral Modulation Therapy for Glioblastoma.
Anticancer Res
; 36(1): 71-80, 2016 Jan.
Article
em En
| MEDLINE
| ID: mdl-26722029
ABSTRACT
BACKGROUND/AIM:
This proof-of-concept study evaluated the antitumor impact of a direct electrical stimulation technique, termed intratumoral modulation therapy (IMT) on glioblastoma (GBM) cells. MATERIALS ANDMETHODS:
An in vitro IMT model comprised of a calibrated electrode to deliver continuous, low-intensity stimulation within GBM preparations. Viability and apoptosis assays were performed in treated immortalized and patient-derived GBM cells, and post-mitotic neurons. IMT was delivered alone and with temozolomide, or gene silencing of the tumor-promoting chaperone, heat-shock protein 27 (HSP27).RESULTS:
GBM cells, but not neurons, exhibited >40% loss of viability, caspase-3 activation and apoptosis with IMT. Cell death was modest with temozolomide alone (30%) but increased significantly with concomitant IMT (70%). HSP27 silencing alone produced 30% viability loss, with significant enhancement of target knockdown and GBM cell death (65%), when combined with IMT.CONCLUSION:
These findings warrant further evaluation of IMT as a potential novel therapeutic strategy for GBM.Palavras-chave
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Encefálicas
/
Terapia por Estimulação Elétrica
/
Glioblastoma
/
Antineoplásicos Alquilantes
/
Dacarbazina
/
Terapêutica com RNAi
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article