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Anti-inflammatory effects of the butanolic fraction of Byrsonima verbascifolia leaves: Mechanisms involving inhibition of tumor necrosis factor alpha, prostaglandin E(2) production and migration of polymorphonuclear leucocyte in vivo experimentation.
Saldanha, Aline Aparecida; de Siqueira, João Máximo; Castro, Ana Hortência Fonsêca; de Azambuja Ribeiro, Rosy Iara Maciel; de Oliveira, Flávio Martins; de Oliveira Lopes, Débora; Pinto, Flávia Carmo Horta; Silva, Denise Brentan; Soares, Adriana Cristina.
Afiliação
  • Saldanha AA; Pharmacology Laboratory, Federal University of São João del-Rei, 35501-296 Divinópolis, Brazil.
  • de Siqueira JM; Pharmacognosy Laboratory, Federal University of São João del-Rei, 35501-296 Divinópolis, Brazil.
  • Castro AH; Pharmacobotany and Medicinal Plants Laboratory, Federal University of São João del-Rei, 35501-296 Divinópolis, Brazil.
  • de Azambuja Ribeiro RI; Experimental Pathology Laboratory, Federal University of São João del-Rei, 35501-296 Divinópolis, Brazil.
  • de Oliveira FM; Molecular Biology Laboratory, Federal University of São João del-Rei, 35501-296 Divinópolis, Brazil.
  • de Oliveira Lopes D; Molecular Biology Laboratory, Federal University of São João del-Rei, 35501-296 Divinópolis, Brazil.
  • Pinto FC; Department of Natural Sciences, Federal University of São João del-Rei, 36307-352, São João del-Rei, Brazil.
  • Silva DB; Núcleo de Pesquisa em Produtos Naturais e Sintéticos (NPPNS), Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, 14040-903 Ribeirão Preto, Brazil; Laboratório de Produtos Naturais e Espectrometria de Massas (LAPNEM), Federal University of Mato Grosso do Sul, 79070-900 Cam
  • Soares AC; Pharmacology Laboratory, Federal University of São João del-Rei, 35501-296 Divinópolis, Brazil. Electronic address: adrianasouza@ufsj.edu.br.
Int Immunopharmacol ; 31: 123-31, 2016 Feb.
Article em En | MEDLINE | ID: mdl-26724477
The leaves of Byrsonima verbascifolia (Malpighiaceae) are traditionally used to treat various diseases including inflammatory conditions. The main goal of this study was to evaluate the in vivo anti-inflammatory activity of the polar constituents from the butanolic fraction of B. verbascifolia leaves (BvBF), as well as to investigate the mechanisms involved in the anti-inflammatory activity. The polar constituents were identified by liquid chromatography coupled to diode array detector and mass spectrometry (LC-DAD­MS) and matrix-assisted laser desorption/ionization ­ time-of-flight mass spectrometry (MALDI-TOF MS) to obtain a complete chemical profile of the fraction. Forty-five compounds were detected in the BvBF by LC-DAD­MS/MS, including condensed tannins, phenolic acids, flavonoids (flavones and flavonols) and other compounds. In addition, several condensed tannins were identified by MALDI-MS/MS, which are composed predominantly by procyanidin units (PCY) and up to six flavan-3-ol units. The BvBF exhibited significant antioxidant and anti-inflammatory activities. The BvBF inhibited paw edema and polymorphonuclear (PMN) leukocyte migration to the footpad and pleural cavity induced by carrageenan. Furthermore, a minor dose (12.50 mg/kg) of BvBF effectively decreased tumor necrosis factor alpha (TNF-α) and prostaglandin E2 (PGE2) levels in the footpad. These findings suggest that the mechanism of the anti-inflammatory action in the BvBF is linked to the inhibition of the production of inflammatory mediators such as TNF-α and PGE2 and the PMN cell migration.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Anti-Inflamatórios não Esteroides / Malpighiaceae / Edema / Neutrófilos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Anti-Inflamatórios não Esteroides / Malpighiaceae / Edema / Neutrófilos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2016 Tipo de documento: Article