Challenging the Roles of NSP3 and Untranslated Regions in Rotavirus mRNA Translation.
PLoS One
; 11(1): e0145998, 2016.
Article
em En
| MEDLINE
| ID: mdl-26727111
ABSTRACT
Rotavirus NSP3 is a translational surrogate of the PABP-poly(A) complex for rotavirus mRNAs. To further explore the effects of NSP3 and untranslated regions (UTRs) on rotavirus mRNAs translation, we used a quantitative in vivo assay with simultaneous cytoplasmic NSP3 expression (wild-type or deletion mutant) and electroporated rotavirus-like and standard synthetic mRNAs. This assay shows that the last four GACC nucleotides of viral mRNA are essential for efficient translation and that both the NSP3 eIF4G- and RNA-binding domains are required. We also show efficient translation of rotavirus-like mRNAs even with a 5'UTR as short as 5 nucleotides, while more than eleven nucleotides are required for the 3'UTR. Despite the weak requirement for a long 5'UTR, a good AUG environment remains a requirement for rotavirus mRNAs translation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biossíntese de Proteínas
/
RNA Mensageiro
/
Proteínas não Estruturais Virais
/
Regiões 3' não Traduzidas
/
Regiões 5' não Traduzidas
Limite:
Animals
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article