Transgenic FingRs for Live Mapping of Synaptic Dynamics in Genetically-Defined Neurons.
Sci Rep
; 6: 18734, 2016 Jan 05.
Article
em En
| MEDLINE
| ID: mdl-26728131
ABSTRACT
Tools for genetically-determined visualization of synaptic circuits and interactions are necessary to build connectomics of the vertebrate brain and to screen synaptic properties in neurological disease models. Here we develop a transgenic FingR (fibronectin intrabodies generated by mRNA display) technology for monitoring synapses in live zebrafish. We demonstrate FingR labeling of defined excitatory and inhibitory synapses, and show FingR applicability for dissecting synapse dynamics in normal and disease states. Using our system we show that chronic hypoxia, associated with neurological defects in preterm birth, affects dopaminergic neuron synapse number depending on the developmental timing of hypoxia.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sinapses
/
Neurônios
Limite:
Animals
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article