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Flow-Tolerant Adhesion of a Bacterial Pathogen to Human Endothelial Cells Through Interaction With Biglycan.
Salo, Jemiina; Pietikäinen, Annukka; Söderström, Mirva; Auvinen, Kaisa; Salmi, Marko; Ebady, Rhodaba; Moriarty, Tara J; Viljanen, Matti K; Hytönen, Jukka.
Afiliação
  • Salo J; Department of Medical Microbiology and Immunology Turku Doctoral Program for Molecular Medicine.
  • Pietikäinen A; Department of Medical Microbiology and Immunology Turku Doctoral Program for Molecular Medicine.
  • Söderström M; Department of Pathology and Forensic Medicine, Turku University Hospital and University of Turku, Finland.
  • Auvinen K; Department of Medical Microbiology and Immunology MediCity Research Laboratory, University of Turku.
  • Salmi M; Department of Medical Microbiology and Immunology MediCity Research Laboratory, University of Turku.
  • Ebady R; Matrix Dynamics Group, Faculty of Dentistry Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto, Canada.
  • Moriarty TJ; Matrix Dynamics Group, Faculty of Dentistry Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto, Canada.
  • Viljanen MK; Department of Medical Microbiology and Immunology.
  • Hytönen J; Department of Medical Microbiology and Immunology.
J Infect Dis ; 213(10): 1623-31, 2016 May 15.
Article em En | MEDLINE | ID: mdl-26740275
ABSTRACT

BACKGROUND:

Bacterial pathogens causing systemic infections disseminate from the initial infection focus to the target organs usually through the blood vasculature. To be able to colonize various organs, bacteria need to adhere to the endothelial cells of the vascular wall, and the adhesion must be strong enough to resist the shear force of the blood flow.Borrelia burgdorferi sensu lato spirochetes, the causative agents of the tick-borne disease Lyme borreliosis, disseminate hematogenously from the tick bite site to the joints, the heart, and the central nervous system of the patient.

METHODS:

We used both wild-type and genetically modified B. burgdorferi s. l. bacteria, recombinant borrelia adhesins, and an array of adhesion assays carried out both under stationary and flow conditions to investigate the molecular mechanisms of borrelial adhesion to human endothelial cells.

RESULTS:

Borrelia garinii, a member of the B. burgdorferi s. l. complex, adhered to biglycan expressed by human endothelial cells in a flow-tolerant manner. The adhesion was mediated by the decorin-binding protein A (DbpA) and DbpB surface molecules of B. garinii.

CONCLUSIONS:

The proteoglycan biglycan is a receptor molecule for flow-resistant adhesion of the bacterial pathogen B. garinii on human endothelial cells.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aderência Bacteriana / Doença de Lyme / Grupo Borrelia Burgdorferi / Borrelia burgdorferi / Células Endoteliais / Biglicano Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aderência Bacteriana / Doença de Lyme / Grupo Borrelia Burgdorferi / Borrelia burgdorferi / Células Endoteliais / Biglicano Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article