[Identification of Active Composition and Quality Evaluation of Paris Herbs Artificially Cultivated in Different Locations of Three Gorges Reservoir].

ABSTRACT

OBJECTIVE: In order to apply the thesis basis for screening the Paris varieties which was suitable for the environments, the quality of Paris herbs of different varieties cultivated in different locations of Three Gorges Reservoir was evaluated based on the identification of active compositions. METHODS: The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations. RESULTS: Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars. CONCLUSION: It is proved that Paris polyphylla var. yunnanensis and Paris polyphylla var. chinensis could be cultivated on a large scale, but the techniques in artificial cultivation should be studied further. At the same time,Paris bashanensis and Paris polyphylla var. pseudothibetica are good cultivars for planting.