[HPLC Fingerprint of Anisodus tanguticus Root].
Zhong Yao Cai
; 38(5): 957-61, 2015 May.
Article
em Zh
| MEDLINE
| ID: mdl-26767288
ABSTRACT
OBJECTIVE:
To establish an HPLC fingerprint of Anisodus tanguticus root for its quality control.METHODS:
The analysis was carried out on a Ultimate AQ C18 (250 mm x 4.6 mm, 5 µm) column with the gradient elution of acetonitrile and KH2PO4 buffer soution, whose pH was adjusted to 3.0 with phosphoric acid. The flow rate, column temperature, detection wavelength and injection volume was 1.0 mL/min, 30 degrees C, 210 nm and 10 µL separately. The similarity evaluation and principal component analysis were used to analyze HPLC fingerprint of Anisodus tanguticus root.RESULTS:
HPLC fingerprint of Anisodus tanguticus root was established with 15 common peaks by determining 18 batches of Anisodus tanguticus root samples. Four characteristic peaks, anisodine, scopolamine, anisodamine and anisodamine, were confirmed by comparing their retention time and UV spectrum with standard reference substances. The simiarities of 18 batches of Anisodus tanguticus root were between -0.891 and 0.987. Comprehensive evaluation scores of 18 batches of Anisodus tanguticus root were between -0.85 and 0.89 by principal component analysis.CONCLUSION:
The established HPLC fingerprint has good precision, repeatability and stability, which can provide more comprehensive information for identification and quality control of Anisodus tanguticus root.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Medicamentos de Ervas Chinesas
/
Raízes de Plantas
/
Solanaceae
/
Compostos Fitoquímicos
Tipo de estudo:
Prognostic_studies
Idioma:
Zh
Ano de publicação:
2015
Tipo de documento:
Article