Application of the novel bioluminescent ligand-receptor binding assay to relaxin-RXFP1 system for interaction studies.

Wu, Qing-Ping; Zhang, Lei; Shao, Xiao-Xia; Wang, Jia-Hui; Gao, Yu; Xu, Zeng-Guang; Liu, Ya-Li; Guo, Zhan-Yun

Wu, Qing-Ping; Zhang, Lei; Shao, Xiao-Xia; Wang, Jia-Hui; Gao, Yu; Xu, Zeng-Guang; Liu, Ya-Li; Guo, Zhan-Yun.

Afiliação

Wu QP; Research Center for Translational Medicine at East Hospital, College of Life Sciences and Technology, Tongji University, Shanghai, China.
Zhang L; Research Center for Translational Medicine at East Hospital, College of Life Sciences and Technology, Tongji University, Shanghai, China.
Shao XX; Research Center for Translational Medicine at East Hospital, College of Life Sciences and Technology, Tongji University, Shanghai, China.
Wang JH; Research Center for Translational Medicine at East Hospital, College of Life Sciences and Technology, Tongji University, Shanghai, China.
Gao Y; Research Center for Translational Medicine at East Hospital, College of Life Sciences and Technology, Tongji University, Shanghai, China.
Xu ZG; Research Center for Translational Medicine at East Hospital, College of Life Sciences and Technology, Tongji University, Shanghai, China.
Liu YL; Research Center for Translational Medicine at East Hospital, College of Life Sciences and Technology, Tongji University, Shanghai, China. yaliliuliu@163.com.
Guo ZY; Research Center for Translational Medicine at East Hospital, College of Life Sciences and Technology, Tongji University, Shanghai, China. zhan-yun.guo@tongji.edu.cn.

Amino Acids ; 48(4): 1099-1107, 2016 Apr.

Article em En | MEDLINE | ID: mdl-26767372

Assuntos

Bioensaio; Receptores Acoplados a Proteínas G/metabolismo; Receptores de Peptídeos/metabolismo; Relaxina/metabolismo; Sequência de Aminoácidos; Expressão Gênica; Genes Reporter; Vetores Genéticos/química; Vetores Genéticos/metabolismo; Células HEK293; Humanos; Cinética; Ligantes; Luciferases/genética; Luciferases/metabolismo; Medições Luminescentes; Pichia/genética; Pichia/metabolismo; Ligação Proteica; Engenharia de Proteínas; Receptores Acoplados a Proteínas G/genética; Receptores de Peptídeos/genética; Proteínas Recombinantes/genética; Proteínas Recombinantes/metabolismo; Relaxina/genética; Transformação Genética

Palavras-chave

Binding; Bioluminescence; Ligand; RXFP1; Receptor; Relaxin

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Relaxina / Bioensaio / Receptores de Peptídeos / Receptores Acoplados a Proteínas G Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article

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