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BMP6-Engineered MSCs Induce Vertebral Bone Repair in a Pig Model: A Pilot Study.
Pelled, Gadi; Sheyn, Dmitriy; Tawackoli, Wafa; Jun, Deuk Soo; Koh, Youngdo; Su, Susan; Cohn Yakubovich, Doron; Kallai, Ilan; Antebi, Ben; Da, Xiaoyu; Gazit, Zulma; Bae, Hyun; Gazit, Dan.
Afiliação
  • Pelled G; Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Biomedical Imaging Research Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Skeletal Biotec
  • Sheyn D; Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
  • Tawackoli W; Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Biomedical Imaging Research Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
  • Jun DS; Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Department of Orthopaedic Surgery, Gil Medical Center, Gachon University of Medicine & Science, Incheon, Republic of Korea.
  • Koh Y; Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Department of Orthopedics, School of Medicine, Ewha Womans University, Seoul, Republic of Korea.
  • Su S; Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
  • Cohn Yakubovich D; Skeletal Biotech Laboratory, Hadassah Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel.
  • Kallai I; Skeletal Biotech Laboratory, Hadassah Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel.
  • Antebi B; Skeletal Biotech Laboratory, Hadassah Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel; US Army Institute of Surgical Research, Fort Sam Houston, San Antonio, TX 78234, USA.
  • Da X; Biomedical Imaging Research Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
  • Gazit Z; Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Skeletal Biotech Laboratory, Hadassah Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel.
  • Bae H; Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
  • Gazit D; Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Skeletal Biotech Laboratory, Hadassah Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel.
Stem Cells Int ; 2016: 6530624, 2016.
Article em En | MEDLINE | ID: mdl-26770211
ABSTRACT
Osteoporotic patients, incapacitated due to vertebral compression fractures (VCF), suffer grave financial and clinical burden. Current clinical treatments focus on symptoms' management but do not combat the issue at the source. In this pilot study, allogeneic, porcine mesenchymal stem cells, overexpressing the BMP6 gene (MSC-BMP6), were suspended in fibrin gel and implanted into a vertebral defect to investigate their effect on bone regeneration in a clinically relevant, large animal pig model. To check the effect of the BMP6-modified cells on bone regeneration, a fibrin gel only construct was used for comparison. Bone healing was evaluated in vivo at 6 and 12 weeks and ex vivo at 6 months. In vivo CT showed bone regeneration within 6 weeks of implantation in the MSC-BMP6 group while only minor bone formation was seen in the defect site of the control group. After 6 months, ex vivo analysis demonstrated enhanced bone regeneration in the BMP6-MSC group, as compared to control. This preclinical study presents an innovative, potentially minimally invasive, technique that can be used to induce bone regeneration using allogeneic gene modified MSCs and therefore revolutionize current treatment of challenging conditions, such as osteoporosis-related VCFs.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2016 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2016 Tipo de documento: Article