Serial femtosecond X-ray diffraction of enveloped virus microcrystals.
Struct Dyn
; 2(4): 041720, 2015 Jul.
Article
em En
| MEDLINE
| ID: mdl-26798819
ABSTRACT
Serial femtosecond crystallography (SFX) using X-ray free-electron lasers has produced high-resolution, room temperature, time-resolved protein structures. We report preliminary SFX of Sindbis virus, an enveloped icosahedral RNA virus with â¼700 Å diameter. Microcrystals delivered in viscous agarose medium diffracted to â¼40 Å resolution. Small-angle diffuse X-ray scattering overlaid Bragg peaks and analysis suggests this results from molecular transforms of individual particles. Viral proteins undergo structural changes during entry and infection, which could, in principle, be studied with SFX. This is an important step toward determining room temperature structures from virus microcrystals that may enable time-resolved studies of enveloped viruses.
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Coleções:
01-internacional
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MEDLINE
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article