Assessing the Inhibitory Activity of Oligonucleotides on TLR7 Sensing.
Methods Mol Biol
; 1390: 79-90, 2016.
Article
em En
| MEDLINE
| ID: mdl-26803623
ABSTRACT
Aberrant sensing of self-nucleic acids by Toll-like receptor (TLR) 7, 8, or 9 is associated with several autoimmune disorders, including systemic lupus erythematosus (SLE), rheumatoid arthritis, psoriasis, or systemic sclerosis. In recent years, several classes of synthetic oligonucleotides have been shown to antagonize sensing of immunostimulatory nucleic acids by TLR7/8/9, indicating that these molecules could have therapeutic applications in such autoimmune diseases. Conversely, synthetic oligonucleotides used in therapeutic technologies such as antisense and microRNA inhibitors also have the potential to inhibit TLR7/8/9 sensing, rendering patients more susceptible to viral/bacterial infections. This chapter describes a protocol to define the inhibitory activity of synthetic oligonucleotides on TLR7.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligonucleotídeos
/
Receptor 7 Toll-Like
Tipo de estudo:
Guideline
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article