An Expanded Reverse Line Blot Hybridization Protocol for the Simultaneous Detection of Numerous Tick-Borne Pathogens in North America.
J Med Entomol
; 53(3): 721-726, 2016 05.
Article
em En
| MEDLINE
| ID: mdl-26819330
ABSTRACT
Due to an increasing diversity of bacterial pathogens known to be transmitted by hard ticks (Acari Ixodidae) in North America, a comprehensive assay is needed to detect and differentiate among these numerous tick-borne pathogens. We describe an expanded protocol using a combination of multiplex polymerase chain reaction and reverse line blot hybridization to detect a greater diversity of infectious agents than were previously detectable. Ten novel oligonucleotide probes, either individually or in concert, enabled or enhanced identification of six Borrelia species, three Rickettsia species, and one Ehrlichia species. Simultaneous detection of these numerous tick-borne pathogens can advance surveillance efforts and improve accuracy of detection and, thus, reporting.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vetores Aracnídeos
/
Rickettsia
/
Carrapatos
/
Borrelia
/
Doenças Transmitidas por Carrapatos
/
Hibridização In Situ
/
Ehrlichia
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Animals
País/Região como assunto:
America do norte
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article