Actin Dynamics Regulates Dendritic Cell-Mediated Transfer of HIV-1 to T Cells.
Cell
; 164(4): 695-709, 2016 Feb 11.
Article
em En
| MEDLINE
| ID: mdl-26830877
ABSTRACT
Whereas human dendritic cells (DCs) are largely resistant to productive infection with HIV-1, they have a unique ability to take up the virus and transmit it efficiently to T lymphocytes through a process of trans-infection or trans-enhancement. To elucidate the molecular and cell biological mechanism for trans-enhancement, we performed an shRNA screen of several hundred genes involved in organelle and membrane trafficking in immature human monocyte-derived dendritic cells (MDDCs). We identified TSPAN7 and DNM2, which control actin nucleation and stabilization, as having important and distinct roles in limiting HIV-1 endocytosis and in maintaining virus particles on dendrites, which is required for efficient transfer to T lymphocytes. Further characterization of this process may provide insights not only into the role of DCs in transmission and dissemination of HIV-1 but also more broadly into mechanisms controlling capture and internalization of pathogens.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Dendríticas
/
Linfócitos T
/
Infecções por HIV
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Actinas
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HIV-1
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article