Structural and functional analysis of the RNA helicase Prp43 from the thermophilic eukaryote Chaetomium thermophilum.
Acta Crystallogr F Struct Biol Commun
; 72(Pt 2): 112-20, 2016 Feb.
Article
em En
| MEDLINE
| ID: mdl-26841761
ABSTRACT
RNA helicases are indispensable for all organisms in each domain of life and have implications in numerous cellular processes. The DEAH-box RNA helicase Prp43 is involved in pre-mRNA splicing as well as rRNA maturation. Here, the crystal structure of Chaetomium thermophilum Prp43 at 2.9â
Å resolution is revealed. Furthermore, it is demonstrated that Prp43 from C. thermophilum is capable of functionally replacing its orthologue from Saccharomyces cerevisiae in spliceosomal disassembly assays.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
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Proteínas Fúngicas
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Chaetomium
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Cristalografia por Raios X
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RNA Helicases
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Cristalização
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Estabilidade Proteica
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article