Potentiation of radiation-induced bacterial cell killing by binuclear rhodium(II) complexes and their amines.

ABSTRACT

Rhodium(II) binuclear complexes were surveyed for potentiation of radiation-induced cell killing of hypoxic and fully oxic Salmonella typhimurium cells. The Rh2 tetracarbonate ion substantially potentiated hypoxic cell radiation sensitivity. Phosphate interfered with this potentiation. In the latter two respects, radiation potentiation by Rh2 tetracarbonate is similar to that found for Rh2 tetracarboxylates. Amines such as ammonia, methylamine, ethylamine, n-propylamine, and n-butylamine were examined with both Rh2 tetracarbonate and tetraacetate complexes. With Rh2 tetraacetate in phosphate-buffered saline, these amines variably increased radiation potentiation to a maximum of nearly that seen by Rh2 tetraacetate alone in the absence of phosphate. With Rh2 tetracarbonate, particular amines were found to either enhance or restrict radiation potentiation. Results as a whole support the hypothesis that a radiolytic Rh species initiated in a one-electron reduction process external to the cell is responsible for the potentiation by Rh2 complexes in bacteria. Phosphate interference of potentiation by Rh2 tetracetate appears to be limited competitively by amines, suggesting that axial associations of phosphate with the Rh2 center may be involved in the inhibition of radiation potentiation. Of interest in this regard is the finding that 5'-adenosinemonophosphate eliminates the potentiation seen with Rh2 tetraacetate.