Modified RNAs in CRISPR/Cas9: An Old Trick Works Again.
Angew Chem Int Ed Engl
; 55(11): 3548-50, 2016 Mar 07.
Article
em En
| MEDLINE
| ID: mdl-26880106
ABSTRACT
Old tricks, new dog CRISPR/Cas9 is a powerful tool for gene editing that requires an endonuclease (Cas9) and RNA strands. It has been shown that chemical modification of the RNA structures, an approach that has been used to improve the efficiency of RNA interference, can also be applied to enhance the activity of CRISPR/Cas9 and reduce its off-target effects.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA
/
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article