Intercellular Connections Related to Cell-Cell Crosstalk Specifically Recognized by an Aptamer.
Angew Chem Int Ed Engl
; 55(12): 3914-8, 2016 Mar 14.
Article
em En
| MEDLINE
| ID: mdl-26889661
ABSTRACT
Intercellular connections are an important pathway for cell-cell crosstalk. However, their formation mechanism and functions are far from being understood. The lack of molecular probes hampers the research in this area. Herein, we report a kind of intercellular connection that is specifically recognized by aptamer M17A2 generated by cell-SELEX against MCF-7R cells. These connections have different morphologies, but have the same skeleton composed of F-actin. The long filamentous connections were identified to be tunneling nanotubes (TNTs), a recently discovered cell-cell communication route. These connections could be built not only between MCF-7R cells, but also from MCF-7R to other cells after co-culture. Proteins could be transported between cells through these connections, suggesting their cell communication function. Aptamer M17A2 shows the potential to act as a new probe for investigating this kind of intercellular connection, as well as for studying cell-cell communication.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Comunicação Celular
/
Técnica de Seleção de Aptâmeros
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article