One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle.
Sci Rep
; 6: 21953, 2016 Feb 24.
Article
em En
| MEDLINE
| ID: mdl-26905416
ABSTRACT
Genetically modified cattle are important for developing new biomedical models and for an improved understanding of the pathophysiology of zoonotic diseases. However, genome editing and genetic engineering based on somatic cell nuclear transfer suffer from a low overall efficiency. Here, we established a highly efficient one-step multiplex gene transfer system into the bovine genome.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Proteínas de Bactérias
/
Animais Geneticamente Modificados
/
Elementos de DNA Transponíveis
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Genoma
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Transgenes
/
Transposases
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Proteínas Luminescentes
Limite:
Animals
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article