Higher-Density Culture in Human Embryonic Stem Cells Results in DNA Damage and Genome Instability.
Stem Cell Reports
; 6(3): 330-41, 2016 Mar 08.
Article
em En
| MEDLINE
| ID: mdl-26923824
ABSTRACT
Human embryonic stem cells (hESC) show great promise for clinical and research applications, but their well-known proneness to genomic instability hampers the development to their full potential. Here, we demonstrate that medium acidification linked to culture density is the main cause of DNA damage and genomic alterations in hESC grown on feeder layers, and this even in the short time span of a single passage. In line with this, we show that increasing the frequency of the medium refreshments minimizes the levels of DNA damage and genetic instability. Also, we show that cells cultured on laminin-521 do not present this increase in DNA damage when grown at high density, although the (long-term) impact on their genomic stability remains to be elucidated. Our results explain the high levels of genome instability observed over the years by many laboratories worldwide, and show that the development of optimal culture conditions is key to solving this problem.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dano ao DNA
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Técnicas de Cultura de Células
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Instabilidade Genômica
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Células-Tronco Embrionárias
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article