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Comparative Analysis of HIV-1 and Murine Leukemia Virus Three-Dimensional Nuclear Distributions.
Quercioli, Valentina; Di Primio, Cristina; Casini, Antonio; Mulder, Lubbertus C F; Vranckx, Lenard S; Borrenberghs, Doortje; Gijsbers, Rik; Debyser, Zeger; Cereseto, Anna.
Afiliação
  • Quercioli V; Laboratory of Biology, Scuola Normale Superiore, Pisa, Italy.
  • Di Primio C; Laboratory of Biology, Scuola Normale Superiore, Pisa, Italy.
  • Casini A; Laboratory of Molecular Virology, University of Trento, Centre for Integrative Biology, Trento, Italy.
  • Mulder LCF; Department of Microbiology, Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Vranckx LS; Laboratory of Molecular Virology and Gene Therapy, KU Leuven, Leuven, Flanders, Belgium.
  • Borrenberghs D; Laboratory of Molecular Virology and Gene Therapy, KU Leuven, Leuven, Flanders, Belgium.
  • Gijsbers R; Laboratory for Photochemistry and Spectroscopy, Department of Chemistry, KU Leuven, Leuven, Belgium.
  • Debyser Z; Laboratory of Molecular Virology and Gene Therapy, KU Leuven, Leuven, Flanders, Belgium.
  • Cereseto A; Laboratory of Molecular Virology and Gene Therapy, KU Leuven, Leuven, Flanders, Belgium.
J Virol ; 90(10): 5205-5209, 2016 May 15.
Article em En | MEDLINE | ID: mdl-26962222
ABSTRACT
Recent advances in fluorescence microscopy allow three-dimensional analysis of HIV-1 preintegration complexes in the nuclei of infected cells. To extend this investigation to gammaretroviruses, we engineered a fluorescent Moloney murine leukemia virus (MLV) system consisting of MLV-integrase fused to enhanced green fluorescent protein (MLV-IN-EGFP). A comparative analysis of lentiviral (HIV-1) and gammaretroviral (MLV) fluorescent complexes in the nuclei of infected cells revealed their different spatial distributions. This research tool has the potential to achieve new insight into the nuclear biology of these retroviruses.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Núcleo Celular / HIV-1 / Vírus da Leucemia Murina de Moloney Limite: Animals / Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article
Texto completo
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Núcleo Celular / HIV-1 / Vírus da Leucemia Murina de Moloney Limite: Animals / Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article