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[Expression of FAS-AS1 in breast cancer and its effect on the host gene sFas].
Zhou, S W; Su, B B; Zhou, Y.
Afiliação
  • Zhou SW; Department of Head & Neck, and Breast Surgery, Xinxiang Central Hospital, Xinxiang, Henan 453000, China.
Zhonghua Yi Xue Za Zhi ; 96(12): 949-53, 2016 Mar 29.
Article em Zh | MEDLINE | ID: mdl-27045720
ABSTRACT

OBJECTIVE:

To investigate the expression level of antisense transcript of FAS (FAS-antisense 1, FAS-AS1) in breast cancer, and its effect on the host gene soluble Fas receptor (sFas).

METHODS:

The expression of FAS-AS1 and sFas gene was detected in 20 cases of breast cancer tumors and breast cancer cells and their normal counterparts by using real-time quantitative polymerase chain reaction(RT-qPCR). The effects of FAS-AS1 on the expression of sFas gene in breast cancer cells were analyzed by overexpressing FAS-AS1 in breast cancer cells.

RESULTS:

FAS-AS1 expression in breast cancer tissue and cell lines was significantly lower than that of adjacent breast tissue (P=0.000) and normal breast cells (P<0.01). However, sFasexpression in the breast cancer tissue and cell lines was significantly higher than that of adjacent breast tissue (P=0.001) and normal breast cells (P<0.01). The expression of FAS-AS1 was negatively correlated with the expression of sFas(r=-0.751, P=0.020). FAS-AS1 expression was observed to be closely correlated with tumor size (r(2)=0.351, P=0.025) and lymph node metastasis (r(2)=0.265, P=0.043). Expression of sFas and cell proliferation was significantly reduced in FAS-AS1-overexpressing MCF-7 cells (P<0.05).

CONCLUSION:

FAS-AS1 is distinctly downexpressed in breast cancer, and it has certain effects on the expression of host gene sFas and breast cancer development.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias da Mama / Receptor fas / Proteína Ligante Fas / Metástase Linfática Limite: Female / Humans Idioma: Zh Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias da Mama / Receptor fas / Proteína Ligante Fas / Metástase Linfática Limite: Female / Humans Idioma: Zh Ano de publicação: 2016 Tipo de documento: Article