Harvesting epithelial keratinocyte sheets from temperature-responsive dishes preserves basement membrane proteins and improves cell survival in a skin defect model.

Osada, A; Sekine, H; Soejima, K; Sakurai, H; Shimizu, T

Osada, A; Sekine, H; Soejima, K; Sakurai, H; Shimizu, T.

Afiliação

Osada A; Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University (TWIns), Japan.
Sekine H; Department of Plastic and Reconstructive Surgery, Tokyo Women's Medical University, Japan.
Soejima K; Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University (TWIns), Japan.
Sakurai H; Department of Plastic and Reconstructive Surgery, Nihon University School of Medicine, Tokyo, Japan.
Shimizu T; Department of Plastic and Reconstructive Surgery, Tokyo Women's Medical University, Japan.

J Tissue Eng Regen Med ; 11(9): 2516-2524, 2017 09.

Article em En | MEDLINE | ID: mdl-27061496

Assuntos

Membrana Basal/metabolismo; Separação Celular/métodos; Proteínas da Matriz Extracelular/metabolismo; Queratinócitos; Pele; Animais; Sobrevivência Celular; Queratinócitos/metabolismo; Queratinócitos/transplante; Ratos; Ratos Nus; Ratos Sprague-Dawley; Ratos Transgênicos; Pele/lesões; Pele/metabolismo; Pele/patologia

Palavras-chave

basement membrane; cultured epithelial autograft; dispase; survival rate; temperature-responsive culture dish; transplantation

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pele / Membrana Basal / Queratinócitos / Separação Celular / Proteínas da Matriz Extracelular Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article

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