Establishing targeted carp TLR22 gene disruption via homologous recombination using CRISPR/Cas9.
Dev Comp Immunol
; 61: 242-7, 2016 08.
Article
em En
| MEDLINE
| ID: mdl-27079451
ABSTRACT
Recent advances in gene editing techniques have not been exploited in farmed fishes. We established a gene targeting technique, using the CRISPR/Cas9 system in Labeo rohita, a farmed carp (known as rohu). We demonstrated that donor DNA was integrated via homologous recombination (HR) at the site of targeted double-stranded nicks created by CRISPR/Cas9 nuclease. This resulted in the successful disruption of rohu Toll-like receptor 22 (TLR22) gene, involved in innate immunity and exclusively present in teleost fishes and amphibians. The null mutant, thus, generated lacked TLR22 mRNA expression. Altogether, this is the first evidence that the CRISPR/Cas9 system is a highly efficient tool for targeted gene disruption via HR in teleosts for generating model large-bodied farmed fishes.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carpas
/
Proteínas de Peixes
/
Receptores Toll-Like
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Técnicas de Silenciamento de Genes
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Sistemas CRISPR-Cas
Limite:
Animals
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article