A reliable method for avoiding false negative results with Luminex single antigen beads; evidence of the prozone effect.
Transpl Immunol
; 37: 23-27, 2016 07.
Article
em En
| MEDLINE
| ID: mdl-27109036
ABSTRACT
Luminex single antigen bead (SAB) assays have become an essential tool in monitoring the status of antibody to the Human Leucocyte Antigen (HLA) of patients both before and after transplantation. In addition SAB data is used to aid risk stratification to assess immunological risk of humoral rejection in solid organ transplantation (CTAG/BTAG guidelines) [1]. Increasingly laboratories are reporting false negative results at high antibody titre due to a prozone effect. Here we report a case study where the prozone effect led to a false negative antibody result that could have resulted in adverse outcome. We describe a method to reliably remove the prozone effect through heat inactivation and the addition of Ethylenediaminetetraacetic acid (EDTA) to the Luminex wash buffer.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Teste de Histocompatibilidade
/
Antígenos HLA-DQ
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Transplante de Rim
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Reações Falso-Negativas
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Glomerulonefrite por IGA
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Rejeição de Enxerto
/
Isoanticorpos
Tipo de estudo:
Etiology_studies
/
Risk_factors_studies
Limite:
Adult
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Humans
/
Male
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article