Cordyceps militaris induces tumor cell death via the caspasedependent mitochondrial pathway in HepG2 and MCF7 cells.
Mol Med Rep
; 13(6): 5132-40, 2016 Jun.
Article
em En
| MEDLINE
| ID: mdl-27109250
ABSTRACT
Cordyceps militaris (CM), an entomopathogenic fungus belonging to the class ascomycetes, possesses various pharmacological activities, including cytotoxic effects, on various types of human tumor cells. The present study investigated the antihepatocellular carcinoma (HCC) and antibreast cancer effects of CM in in vitro and in vivo models. CM aqueous extract reduced cell viability, suppressed cell proliferation, inhibited cell migration ability, caused the over-release of lactate dehydrogenase, induced mitochondrial dysfunction and enhanced apoptotic rates in MCF7 and HepG2 cells. The expression levels of cleaved poly (ADP ribose) polymerase and caspase3, biomarkers of apoptosis, were increased following treatment with CM aqueous extract for 24 h. Furthermore, in the MCF7 and HepG2 cells, enhanced levels of B cellassociated X protein and cleaved caspase8 were observed in the CMtreated cells. Finally, the antitumor activities of CM in HCC and breast cancer were also confirmed in MCF7 and HepG2xengraft nude mice models. Collectively, the data obtained in the present study suggested that the cytotoxic effects of CM aqueous extract on HCC and breast cancer are associated with the caspasedependent mitochondrial pathway.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
/
Mitocôndrias Hepáticas
/
Apoptose
/
Carcinoma Hepatocelular
/
Proteínas Mitocondriais
/
Cordyceps
/
Misturas Complexas
/
Neoplasias Hepáticas
/
Proteínas de Neoplasias
Limite:
Animals
/
Female
/
Humans
/
Male
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article