Timing embryo biopsy for PGD - before or after cryopreservation?
Gynecol Endocrinol
; 32(9): 756-758, 2016 Sep.
Article
em En
| MEDLINE
| ID: mdl-27113862
ABSTRACT
OBJECTIVE:
Pre-implantation genetic diagnosis (PGD) is required in order to screen and diagnose embryos of patients at risk of having a genetically affected offspring. A biopsy to diagnose the genetic profile of the embryo may be performed either before or after cryopreservation. The aim of this study was to determine which biopsy timing yields higher embryo survival rates. STUDYDESIGN:
Retrospective cohort study of all PGD patients in a public IVF unit between 2010 and 2013. Inclusion criteria were patients with good-quality embryos available for cryopreservation by the slow freezing method. Embryos were divided into two groups biopsy before and biopsy after cryopreservation. The primary outcome was embryo survival rates post thawing.RESULTS:
Sixty-five patients met inclusion criteria. 145 embryos were biopsied before cryopreservation and 228 embryos were cryopreserved and biopsied after thawing. Embryo survival was significantly greater in the latter group (77% vs. 68%, p < 0.0001).CONCLUSION:
Cryopreservation preceding biopsy results in better embryo survival compared to biopsy before cryopreservation.Palavras-chave
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biópsia
/
Criopreservação
/
Diagnóstico Pré-Implantação
/
Técnicas de Reprodução Assistida
/
Transferência Embrionária
Tipo de estudo:
Diagnostic_studies
/
Observational_studies
Limite:
Adult
/
Female
/
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article