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Diagnosis of tuberculosis based on the detection of a cocktail of mycobacterial antigen 85B, ESAT-6 and cord factor by immuno-PCR.
Mehta, Promod K; Singh, Netrapal; Dharra, Renu; Dahiya, Bhawna; Sharma, Suman; Sheoran, Abhishek; Gupta, Krishna B; Chaudhary, Dhruva; Mehta, Neeru; Varma-Basil, Mandira.
Afiliação
  • Mehta PK; Centre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak, 124001 Haryana, India. Electronic address: pkmehta3@hotmail.com.
  • Singh N; Centre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak, 124001 Haryana, India.
  • Dharra R; Centre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak, 124001 Haryana, India.
  • Dahiya B; Centre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak, 124001 Haryana, India.
  • Sharma S; Centre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak, 124001 Haryana, India.
  • Sheoran A; Department of Statistics, MDU, Rohtak, India.
  • Gupta KB; Department of TB and Respiratory Medicine, University of Health Sciences (UHS), Rohtak, India.
  • Chaudhary D; Pulmonary and Critical Care Medicine, UHS, Rohtak, India.
  • Mehta N; Department of Medical Electronics, Ambedkar Institute of Technology, Shakarpur, Delhi 110092, India.
  • Varma-Basil M; Vallabhbhai Patel Chest Institute (VPCI), University of Delhi, Delhi 110007, India.
J Microbiol Methods ; 127: 24-27, 2016 08.
Article em En | MEDLINE | ID: mdl-27164021
ABSTRACT
Attempts were made to enhance the sensitivity of immuno-PCR assay based on the detection of cocktail of mycobacterial antigen 85B (Rv1886c), ESAT-6 (Rv3875) and cord factor (trehalose 6,6'-dimycolate) in pulmonary and extrapulmonary TB patients. Detection of Ag85B was found to be superior to the detection of cocktail in TB patients.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tuberculose / Reação em Cadeia da Polimerase / Fatores Corda / Antígenos de Bactérias Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2016 Tipo de documento: Article
Texto completo
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PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tuberculose / Reação em Cadeia da Polimerase / Fatores Corda / Antígenos de Bactérias Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2016 Tipo de documento: Article