Syndecan-4, a PRRSV attachment factor, mediates PRRSV entry through its interaction with EGFR.
Biochem Biophys Res Commun
; 475(2): 230-7, 2016 06 24.
Article
em En
| MEDLINE
| ID: mdl-27208778
ABSTRACT
The causative agent of porcine reproductive and respiratory syndrome is the PRRS virus (PRRSV), an enveloped, single-stranded and positive-sense RNA virus. The host factors and mechanisms that are involved in PRRSV entry are still largely unknown. In our present studies, we found that syndecan-4, one of the heparan sulfate proteoglycans, plays a critical role in PRRSV entry, especially in PRRSV attachment. Moreover, EGFR interacts with syndecan-4 in MACR-145 cells and disruption of their interaction impaired PRRSV entry. Furthermore, EGFR inhibitor AG1478 or syndecan-4 derived peptide SSTN87-131 inhibited syndecan-4 endocytosis induced by PRRSV entry. Altogether, syndecan-4, a PRRSV attachment factor, mediated PRRSV entry by interacting with EGFR.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Suínos
/
Vírus da Síndrome Respiratória e Reprodutiva Suína
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Síndrome Respiratória e Reprodutiva Suína
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Sindecana-4
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Receptores ErbB
Limite:
Animals
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article