Reporter Systems to Study Cancer Stem Cells.

Saygin, Caner; Samour, Mohamed; Chumakova, Anastasia; Jarrar, Awad; Lathia, Justin D; Reizes, Ofer

Saygin, Caner; Samour, Mohamed; Chumakova, Anastasia; Jarrar, Awad; Lathia, Justin D; Reizes, Ofer.

Afiliação

Saygin C; Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, NC 10, Cleveland, OH, 44195, USA.
Samour M; Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, NC 10, Cleveland, OH, 44195, USA.
Chumakova A; Medicine Institute, Cleveland Clinic, Cleveland, OH, 44195, USA.
Jarrar A; Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, NC 10, Cleveland, OH, 44195, USA.
Lathia JD; Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, NC 10, Cleveland, OH, 44195, USA.
Reizes O; Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, NC 10, Cleveland, OH, 44195, USA.

Methods Mol Biol ; 1516: 319-333, 2016.

Article em En | MEDLINE | ID: mdl-27221339

RESUMO

Cancer stem cells have been identified in primary tumors, patient derived xenografts, and established cancer cell lines. The development of reporters has enabled investigators to rapidly enrich for these cells and more importantly track these cells in real time. Here we describe the current state of the reporter field and their use and limitations in multiple cancers.

Assuntos

Genes Reporter/genética; Biologia Molecular/métodos; Neoplasias/genética; Células-Tronco Neoplásicas/metabolismo; Células-Tronco Embrionárias/citologia; Células-Tronco Embrionárias/metabolismo; Humanos; Proteína Homeobox Nanog/genética; Proteína Homeobox Nanog/metabolismo; Neoplasias/patologia; Células-Tronco Neoplásicas/patologia

Palavras-chave

Cancer stem cells; NANOG; Pluripotency transcription factor; Reporter; Tumor heterogeneity

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco Neoplásicas / Genes Reporter / Biologia Molecular / Neoplasias Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article

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