Effect of melatonin supplementation on cryopreserved sperm quality in mouse.
Cryo Letters
; 37(2): 115-22, 2016.
Article
em En
| MEDLINE
| ID: mdl-27224523
ABSTRACT
BACKGROUND:
Antioxidants protect spermatozoa against cell damage during cryopreservation.OBJECTIVE:
To investigate whether melatonin supplement in the extender may improve the quality of cryopreserved mouse sperm.METHODS:
Kunming mice sperm frozen in extender R18S3 (18% (w/v) rafï¬nose and 3% (w/v) skim milk) supplemented with melatonin were thawed and evaluated.RESULTS:
Mouse spermatozoa were cryopreserved in the freezing extender R18S3 that contained melatonin at 0, 0.125, 0.25 and 0.5 mg/mL melatonin. The extender without melatonin supplement was associated with increased formation of reactive oxygen species (ROS) and decreased sperm motility. Melatonin supplement at 0.125 mg/mL significantly increased the progressive motility of sperm in comparison to other melatonin concentration or control. The percentage of thawed viable sperm with ROS was lower in the melatonin-treated groups than in untreated group. Melatonin supplement also increased antiapoptotic gene Bcl-xl expression in the thawed sperm.CONCLUSION:
Supplement of 0.125 mg/mL melatonin could reduce oxidative damage and apoptosis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Preservação do Sêmen
/
Espermatozoides
/
Criopreservação
/
Crioprotetores
/
Melatonina
Limite:
Animals
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article